NanoDrop™ Spectrophotometer Exchange

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NanoDrop™ Spectrophotometer Exchange


Just some of the many highlights of the NEW NanoDrop™ One include:

  • ♦ Acclaro™ sample intelligence – Contaminant Detection
  • ♦ Compact standalone design – Reduce Bench Space impact
  • ♦ Auto blank – Auto measure – Improve your Workflow
  • ♦ Detect Bubbles – Improve Measurement Integrity


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Product Description

Hardware Features

NanoDrop™ 1000 NanoDrop™ 2000/2000c NanoDrop™ One/OneC
Microvolume sampling
PC Software
Fast and Easy to use ‘pipette, measure, clean”
Xenon flash lamp light source
Dynamic range (ng / µL dsDNA) 2-3,750 0.4-15,000 0.2-27,500
No sample carryover
Measurement time (s) 10 5 typical 6
Multiple auto-range pathlengths 2 4 5
Wavelength range (nm) 220-750 190-840 190-850
Spectral resolution (nm, FWHM) <3 at 546nm <1.8 at 254nm <1.8 at 254nm
Cuvette option with stirring and heating
Spectrograph with enhanced UV and stray light control
Spectrograph with native low-stray light and deep UV detection
Precision short-pathlength control
Stand alone instrument with local control
High-resolution, adjustable glove-compatible touchscreen
Enhanced connectivity: Ethernet, Wi-Fi and Bluetooth
Cuvette can be used with arm up or down

Software Features

NanoDrop™ 1000 NanoDrop™ 2000/2000c NanoDrop™ One/OneC
Nucleic Acid A260
Nucleic Acid purity ratio A260/A280, A260/A230
Protein A280
Protein Purity ratio A260/280
Protein colorimetric assays: BCA and Bradford
Proteins and Labels
Cell Culture OD600
Kinetics measurements
Create and save custom methods
Protein colorimetric assays: Lowry and Pierce 660
Auto-Blank and Auto-Measure capability
Protein Editor for adding user protein
Protein A205 method
Preconfigured custom methods for nanoparticles, chlorophyll and hemoglobin
Acclaro Embedded Technical support
Acclaro Onboard Learning
Acclaro Sample integrity: digital image processing

Click here to see the NanoDrop Internal Comparison Chart


NanoDrop One
NanoDrop One - Illustration

Product Description

In 2001 the NanoDrop™ ND1000 became the world’s first microvolume spectrophotometer. It introduced scientists to the benefits of the now patented NanoDrop™ sample retention technology. Users simply had to pipette microliter amounts of sample onto the pedestal, measure and then wipe. The large dynamic range meant there was no need for dilutions and the software took care of all calculations. Over the last 15 years the ND1000 has become the quantification instrument of choice for researchers across the world.

In December 2017 however the ND1000 will be officially retired, meaning that factory software and hardware support will formally end. In order to help owners of the ND1000 NanoDrop™ to upgrade, we are offering a very generous trade in allowance against the latest Thermo Scientific™ NanoDrop™ One range. The NanoDrop™ One includes everything you loved about your ND1000 together with many new features to enhance your research:

  • A compact standalone design with no need for a separate PC, saves valuable bench space
  • New software features, including auto blank and auto measure improve your workflow
  • Increase confidence in your data with unique Acclaro™ sample intelligence technology, which enables you to
  • Identify key contaminants in your samples and obtain corrected concentrations
  • Be alerted to issues with your blank or your sample’s purity ratios
  • Detect bubbles and anomalies that can affect measurement integrity
  • Learn from guided troubleshooting and on-board videos
  • Data is automatically saved in real time and can be exported in a range of formats
  • The newly increased dynamic and wavelength ranges provide greater assay flexibility, for example to allow quantification of peptides at 205 nanometres
  • For even greater assay flexibility, you can choose the NanoDrop One C model which includes an integrated cuvette reader with heater and magnetic stirrer

NanoDrop One FAQ

How are NanoDrop™ spectrophotometers different from traditional cuvette-based instruments?

NanoDrop spectrophotometers use a patented microvolume sample retention system to minimise sample consumption and eliminate the need for cuvettes. A variable, auto-range pathlength feature allows users to measure up to 366x higher sample concentrations than can be measured in a 10 mm cuvette making dilution steps unnecessary.

How are the Thermo Scientific™ NanoDrop™ One Spectrophotometers different from the NanoDrop™ 2000?

The NanoDrop™ One is the next generation instrument in the NanoDrop™ series of microvolume UV-Vis Spectrophotometers and will replace the NanoDrop2000™ and 2000C. The NanoDrop™ One does everything that its predecessors can and more, including: on-board instrument control via a touch screen display, a broader dynamic range (up to 27,500 ng/ µL dsDNA), expanded connectivity and data management options, and enhanced sample analysis and technical support with Acclaro™ Sample Intelligence technology – see Internal Comparison Document.

What is Acclaro™ Sample Intelligence technology?

Thermo Scientific™ Acclaro™ Sample Intelligence technology enhances user understanding of sample quality while delivering accurate quantitative measurements. There are three parts to Acclaro™: (i) data analysis algorithms that provide contaminant identification and corrected concentrations, (ii) an embedded sensor and digital image analysis that monitors the sample column for bubbles to ensure measurement integrity and (iii) sample information alerts and on-demand technical support for guided troubleshooting.

Which contaminants does Acclaro™ detect?

In DNA samples, Acclaro™ detects the following contaminants: proteins, phenol, Guanidine HCl and Guanidine Isothiocyanate. In protein samples (direct A280 measurements) Acclaro™ can detect nucleic acids and phenol. More contaminants will be offered in future software updates.

How does Acclaro™ help you troubleshoot?

When the user taps the information icon on the measurement screen, a popup box will report the sample issue detected and offer possible causes and solutions. If the user wants to “Learn More” they will be guided through a cascade of information, from fundamentals to the specifics. This is best explained with an example. When  Acclaro™ detects a low A260/A280result, a popup box will inform that the ratio is outside acceptable limits for pure DNA sample and that this could be due to a poor blank or a contaminant. The user will then see information on common contaminants that can affect this ratio, and other resources starting with an animation that explains “What is a purity ratio.” If protein is the more likely contaminant, s/he can look at spectra of DNA/ protein samples and how increasing protein levels can affect the purity ratios of a DNA sample. Is another contaminant more likely?

What is the difference between the NanoDrop™ One and the NanoDrop™ OneC?

Both models have the patented microvolume sample retention capability (pedestal). In addition, the NanoDrop™ OneC has a built-in cuvette capability which allows for all the same pedestal applications plus cuvette measurements to support kinetics applications and dilute sample measurements. The cuvette position can be used with the arm up or down and comes with temperature control and stirring features.

What pre-configured applications are included in the NanoDrop™ One/OneC?

Nucleic Acid A260 (ds DNA, ssDNA, oligo DNA, oligo RNA, custom factor) , A260/280, A260/230 and Microarray (labeled nucleic acids); Protein A280 and A205, Protein Pierce 660, Protein Bradford, protein BCA, Protein Lowry, Proteins and labels; OD600; Kinetics, UV-Vis and Custom Methods.

Is there an ability to create and save custom methods?

Yes. Methods can be built with customised analysis wavelengths, factors, extinction coefficients and more. Fluorescent labels can be saved in the Dye Chromophore editor and new protein methods can be saved in the Protein Editor.

Is simply wiping the pedestal surfaces enough to prevent carryover?

Yes. The highly polished quartz and stainless steel surfaces of the sample retention system are resistant to sample adherence, making the use of dry lab wipes very effective in removing the sample.

Can I verify that my instrument is calibrated and running accurately?

Yes. While most users will never need to re-calibrate their NanoDrop™ One, we recommend verifying instrument performance using the NanoDrop™ One performance verification solution (PV-1) every 6 months. PV-1 is available from Thermo Fisher Scientific™ and authorised dealers.

Does the NanoDrop™ One/OneC require a computer to operate?

No. These instruments feature an on-board, high resolution touchscreen control with an Android-based operating system and 32 GB flash memory. USB, Ethernet and WiFi connectivity options allow seamless data transfer to an external computer with Windows® 7 or 10, 64-bit operating systems. The instrument can only be controlled from the touchscreen. Customers can download the NanoDrop One PC software from our website to view and analyse their data, and explore Acclaro™ support features at their desks.

Can I measure purified and unpurified proteins on the NanoDrop™ One/OneC ?

Purified proteins can be measured directly using the A280 or A205 pre-configured applications. Choose the most suitable extinction coefficient from the pre-defined protein sample types (BSA, IgG, Lysozyme) from our drop-down list or add your own custom protein using the Protein Editor feature. For unpurified proteins or cell lysates, a selection of pre-configured colorimetric assay applications is available.

What sort of measurement performance should I expect with these instruments?

The photometric accuracy is within 3% at 0.97A at 302 nm. Typical measurement reproducibility is 0.002A (1.0 mm path) or 1% CV, whichever is greater.


Click here to see the NanoDrop One FAQs

Nucleic Acids

  • Simple operation- Pipette, Measure, Clean (as with the ND1000)
  • No specialist consumables required
  • Wide dynamic range 2 – 27,500 ng/ml ds DNA means no requirement for dilutions. The maximum concentration is ~8 fold higher than on the ND1000
  • 5 Auto ranging pathlengths ensure accurate data across the whole concentration range with no prior knowledge of sample concentration required
  • Automatic calculation of sample concentration, A260/A280 and A260/A230 Purity ratios
  • Dedicated Oligo calculation software module
  • Microarray module for labelling efficiency determination
  • Acclaro Sample Intelligence Technology
    • Identification of key contaminants with corrected concentration
    • Information alerts for abnormal purity ratios and issues with the blank
    • Digital image processing alerts to bubbles and anomalies with the sample that can affect measurement integrity
    • On-board guided troubleshooting and learning


Nucleic Acid Performance Data
Acclaro Contaminant Identification FAQs
Acclaro Protein Contaminant ID
Acclaro Phenol Contaminant ID

Proteins and Peptides

  • Simple operation-Pipette, Measure, Clean (as with the ND1000)
  • No specialist consumables required
  • Wide dynamic range 0.06- 820 mg/ml BSA means no requirement for dilutions. The maximum concentration is ~8 fold higher than on the ND1000
  • 5 Auto ranging pathlengths ensure accurate data across the whole concentration range no prior knowledge of sample concentration required
  • Automatic calculation of sample concentration, A260/A280 and Purity ratio
  • Protein Editor allows input/saving of user defined protein information
  • Dedicated A205 module for quantification of peptides and proteins lacking tryptophan or tyrosine residues
  • Spectrograph with native low stray light and deep UV detection
  • Microarray module for labelling efficiency determination
  • Pre-configured modules for colorimetric protein assays including Bradford, BCA, Lowry and Pierce 660
  • Acclaro™ Sample Intelligence Technology
    • Identification of key contaminants with corrected concentration
    • Information alerts for abnormal purity ratios and issues with the blank
    • Digital image processing alerts to bubbles and anomalies with the sample that can affect measurement integrity
    • On-board guided troubleshooting and learning


Protein and Peptide Quantification at 205nm

Design and Operation

  • Compact stand alone design (no external PC required)
  • Auto blank and auto measure features streamline your workflow
  • Large glove compatible touchscreen interface with tilt – slide capability to ensure ergonomic operation
  • 3 USB ports for connectivity to external input devices (keyboard, mouse, barcode reader), USB flash drives or printer
  • Bluetooth connectivity for external keyboard
  • Wifi and Ethernet connectivity
  • Multi-language support
  • The NanoDrop™ OneC model also includes an integrated cuvette reader with:
    • Heater and magnetic stirrer
    • The ability to use with arm up or down
    • Extended sensitivity (down to 0.2 ng/µl ds DNA and 0.006 mg/ml BSA)
    • Increased assay flexibility for OD measurements, protein assays, kinetics or sample in solvents

Data Handling

  • Real time saving of results
  • Export in a range of formats including .csv and .tsv
  • Export of data via USB flash drive, Ethernet or Wifi
  • Free PC Data Viewer software allows you to visualise data, spectra and Acclaro™ information offline
  • Intuitive search functionality with User Identifiers
  • Connect a Dymo Labelwriter 450 for lab notebook friendly adhesive label printouts
  • Printing of spectral data or reports to Wi-Fi network printers or USB connected printers

Other Applications

  • Full wavelength scans from 190-850 nm using the UV-Vis module
  • OD 600 measurements with cell number conversion option
  • Create your own Custom Methods using the free PC Data Viewer software
  • Download pre-configured methods including cholorophyll and gold nanoparticles
  • For the NanoDrop™ One model perform kinetic assays using the integrated cuvette reader with heater and magnetic stirrer


File Type
Brochure for NanoDrop One PDF
Compare the Nanodrop range PDF


Instrument Control Built-in touchscreen
Minimum Sample Volume 1 µL
Limit of Detection 
Pedestal: 2.0 ng/µL Cuvette: 0.2 ng/µL
BSA (IgG) Pedestal: 0.06 (0.03) mg/mL  Cuvette: 0.006 (0.003) mg/mL
Maximum Concentration
Pedestal: 27,500 ng/µL
BSA (IgG) Pedestal: 820 (400) mg/mL
Measurement and Data Processing Time 8 seconds
Measurement Repeatability 1 Typical: 0.002 A (1.0 mm path)  or 1%CV, whichever is greater
Range 190–850 nm
Accuracy ±1 nm
Range (10 mm equivalent) Pedestal: 0–550 A Cuvette: 0–1.5 A
Accuracy 2 3% at 0.97 A, 302 nm
Resolution (Spectral Bandwidth) ≤1.8 nm (FWHM at Hg 254 nm)
Pathlength 0.030 to 1.0 mm auto-ranging
Light Source Xenon flash lamp
Detector 2048-element CMOS linear  image sensor
Dimensions (W × D × H) 20 × 25.4 × 32.3 cm
(8 × 10 × 12.7 in.)
Weight 3.6 kg (7.9 lbs.)
Operating Voltage 12v (DC)
Power Consumption Operating: 12–18w Standby: 5w
Stirring (cuvette only) 9 speeds
Temperature Control (cuvette only) 37 °C
On-Board Control Operating System Android™
CPU Quad Core ARM® Cortex™-A9 Processor
Display 7-inch, 1280 × 800 high-definition  color display
Touchscreen Multipoint capacitive touch
Gesture Recognition Single point, single point hold, swipe  and pinch
Glove Compatibility Compatible with lab gloves
Internal Storage 32 GB flash memory
Audio Built-in speaker
Connectivity Three USB-A ports, Ethernet, Bluetooth® and WiFi 3
PC Software Requirements Windows® 7 and 10, 64 bit
Accessory Support DYMO® LabelWriter® 450 printer, Bluetooth keyboard,  mouse and barcode reader
Applications Support Nucleic Acid A260, A260/A280, A260/A230 and Labeled
Nucleic Acids; Protein A280 and A205, Protein Pierce 660,
Protein Bradford, Protein BCA, Protein Lowry, Labeled Proteins,
OD600, Kinetics, UV-Vis, and Custom Methods
Language  Support
Chinese, French, German,Japanese, Korean, Polish, Spanish, English

1 – SD of 10 individual measurements at 0.97A

2 – Absorbance expressed at Abs/mm at 25 °C

3 – Only available on instruments with WiFi/Bluetooth support