The UCE17 Novallele Genotyping Assay is designed to function as a positive control for high-resolution melting (HRM) analysis and will generate a single melt peak that is consistent across all human samples. An ultraconserved element (UCE) is a region of at least 200 base pairs that are identical across human, rat, and mouse genomes. UCE detection is commonly used to establish evolutionary relationships among taxa.
The UGT2B15 c.253G>T Novallele Genotyping Assay identifies a single nucleotide change within the UGT2B15 gene. Mutations of the UGT2B15 gene may be associated with UGT2B15 drug-related metabolism and estrogen receptors involved in breast cancer. Mutations in the UGT2B15 may affect metabolism of lorazepam and oxazepam.
The VCAN c.5808T>C Novallele Genotyping Assay identifies a single nucleotide change of the VCAN gene. The VCAN gene encodes for a protein involved in cell adhesion, proliferation, migration, and angiogenesis. Research has shown mutations in the VCAN gene may be associated with Wagner syndrome type 1.
The VKORC1 c.-1639G>A Novallele Genotyping Assay identifies a single mutation associated with the VKORC1 gene. Mutations of the VKORC1 gene cause complete resistance to warfarin, characterized by increased bleeding tendency and thromboembolic diseases like pulmonary embolism and stroke.